E8140
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 | Leukocyte elastase is a 29 kDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds. It contains two or thee N-linked glycans of variable composition which account for its three major isoforms. |
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E8140
Sigma
Elastase from human leukocytes
lyophilized powder, ≥50 units/mg protein (Bradford)
| Synonym: | Lysosomal elastase |
| CAS Number: | 9004-06-2 |
| Enzyme Commission (EC) Number: | 3.4.21.37 ( BRENDA | IUBMB ) |
| MDL number: | MFCD00130997 |
Description
| Biochem/physiol Actions | Unlike pancreatic elastase the leukocyte enzyme has a preferential cleavage for the carboxyl side of valine, but will also cleave to a lesser extent after alanine. Natural substrates include elastin, cartilage proteoglycans, collagen types I, II, II and IV, and fibronectin. |
| Unit Definition | One unit will release one nanomole of p-nitrophenol per sec from BOC- |
| Physical form | Lyophilized from 0.5 M pyridinium acetate, pH 5.3 |
| Physical properties | Leukocyte elastase is a 29 kDa serine endoprotease of the Proteinase S1 Family. It exists as a single 238 amino acid-peptide chain with four disulfide bonds. It contains two or thee N-linked glycans of variable composition which account for its three major isoforms. Isoelectric point: pI = 8.77 - 9.55 |
Properties
| form | lyophilized powder |
| Gene Information | human ... ELA2(1991) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| Hazard Codes | B |
| WGK Germany | 3 |
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